Afficher la couverture 'Ligand-dependent contribution of RXRbeta to cholesterol homeostasis in Sertoli cells | Mascrez, Bénédicte' en grand format
/5

0 avis

Ligand-dependent contribution of RXRbeta to cholesterol homeostasis in Sertoli cells

Archive ouverte

Mascrez, Bénédicte | Ghyselinck, Norbert | Watanabe, Mitsuhiro | Annicotte, Jean-Sébastien | Chambon, Pierre | Auwerx, Johan | Mark, Manuel

Edité par CCSD ; EMBO Press -

We show that mice expressing retinoid X receptor beta (RXRbeta) impaired in its transcriptional activation function AF-2 (Rxrb(af20) mutation) do not display the spermatid release defects observed in RXRbeta-null mutants, indicating that the role of RXRbeta in spermatid release is ligand-independent. In contrast, like RXRbeta-null mutants, Rxrb(af20) mice accumulate cholesteryl esters in Sertoli cells (SCs) due to reduced ABCA1 transporter-mediated cholesterol efflux. We provide genetic and molecular evidence that cholesterol homeostasis in SCs does not require PPARalpha and beta, but depends upon the TIF2 coactivator and RXRbeta/LXRbeta heterodimers, in which RXRbeta AF-2 is transcriptionally active. Our results also indicate that RXRbeta may be activated by a ligand distinct from 9-cis retinoic acid.

Consulter en ligne

Suggestions

Du même auteur

A genetic dissection of the retinoid signalling pathway in the mouse

Archive ouverte | Mark, Manuel | CCSD

To determine the functions of retinoic acid receptors RAR and RXR, we have systematically knocked-out their genes by homologous recombination in the embryonic stem cells and generated null-mutant mice. This approach has allowed us...

Mesectoderm is a major target of retinoic acid action

Archive ouverte | Mark, Manuel | CCSD

International audience. The RAR and RXR families of retinoid nuclear receptors each comprise three isotypes (alpha, beta and gamma). In vitro, RARs bind to their cognate DNA response elements as heterodimers with RX...

Genetic and pharmacological evidence that a retinoic acid cannot be the RXR-activating ligand in mouse epidermis keratinocytes

Archive ouverte | Calléja, Cécile | CCSD

International audience. Using genetic and pharmacological approaches, we demonstrate that both RARγ/RXRα heterodimers involved in repression events, as well as PPARβ(δ)/RXRα heterodimers involved in activation event...

Chargement des enrichissements...