Structural insights into the evolution of late steps of translation initiation in the three domains of life

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Kazan, Ramy | Bourgeois, Gabrielle | Lazennec-Schurdevin, Christine | Coureux, Pierre-Damien | Mechulam, Yves | Schmitt, Emmanuelle

Edité par CCSD ; Elsevier -

International audience. In eukaryotes and in archaea late steps of translation initiation involve the two initiation factors e/aIF5B and e/aIF1A. These two factors are also orthologous to the bacterial IF2 and IF1 proteins, respectively. Recent cryo-EM studies showed how e/aIF5B and e/aIF1A cooperate on the small ribosomal subunit to favor the binding of the large ribosomal subunit and the formation of a ribosome competent for elongation. In this review, pioneering studies and recent biochemical and structural results providing new insights into the role of a/eIF5B in archaea and eukaryotes will be presented. Recent structures will also be compared to orthologous bacterial initiation complexes to highlight domain-specific features and the evolution of initiation mechanisms.

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