X-ray Crystal Structures of 70 S Ribosome Functional Complexes

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Cate, Jamie | Yusupov, Marat | Yusupova, Gulnara Zh. | Earnest, Thomas | Noller, Harry

Edité par CCSD ; American Association for the Advancement of Science (AAAS) -

International audience. Structures of 70 S ribosome complexes containing messenger RNA and transfer RNA (tRNA), or tRNA analogs, have been solved by x-ray crystallography at up to 7.8 angstrom resolution. Many details of the interactions between tRNA and the ribosome, and of the packing arrangements of ribosomal RNA (rRNA) helices in and between the ribosomal subunits, can be seen. Numerous contacts are made between the 30 S subunit and the P-tRNA anticodon stem-loop; in contrast, the anticodon region of A-tRNA is much more exposed. A complex network of molecular interactions suggestive of a functional relay is centered around the long penultimate stem of 16 S rRNA at the subunit interface, including interactions involving the “switch” helix and decoding site of 16 S rRNA, and RNA bridges from the 50 S subunit.

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