Molecular cloning of mRNA sequences transiently induced during rat liver regeneration*1

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Sobczak, Joelle | Lotti, Anne-Marie | Taroux, Philippe | Duguet, Michel

Edité par CCSD ; Elsevier -

International audience. In order to isolate genes which are induced during liver regeneration, we have constructed a cDNA library from 16-h-regenerating liver poly(A)+ RNA. By computer analysis of autoradiograms produced by differential plaque hybridization with cDNA from normal or 16-h-regenerating liver, we have isolated several recombinant clones representing sequences transiently increased during liver regeneration. Three of these were further characterized: the level of the corresponding mRNAs increase rapidly after partial hepatectomy, before the onset of DNA synthesis. Two clones were identified as fibrinogen clones. It is speculated that alpha-fibrinogen may be involved in the growth process, or in its regulation.

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