Fast and accurate identification and antibiotic resistance profiling of micro-organisms in blood cultures by SWATH-MS proteomics

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Macavei, Iulia | Peyrache, Eva | Gregson, Maud | Carrière, Romain | Prat, Roxane | Desbiolles, Chloé | Dufour, Pierre l'Aour | Deforet, Francis | Cecchini, Tiphaine | Dauwalder, Olivier | Vandenesch, François | Lemoine, Jérôme

Edité par CCSD -

International audience. Today, approximately 1.3 million people die each year worldwide from bacterial antimicrobial resistance (AMR). AMR is of great concern in the context of bloodstream infections (BSIs) when it evolves into sepsis. If MALDI-TOF has markedly shortened the delay of the pathogen identification step, AMR evaluation is still based on growth tests in the presence of antibiotics. The latter, negatively impacts the diagnosis turnaround time, thus the administration to the patient of active antimicrobial agents. With the aim of streamlining this turnaround time to less than an hour from a positive blood culture, the LC/SWATH-MS sensitivity and specificity performances were evaluated to concomitantly provide the identity and antibiotic resistance profile of pathogens related to BSIs based on peptide surrogates.

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