Syracuse

Decrease in non-classical monocytes in Myasthenia Gravis patients

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Hemery, Edouard | Truffault, Frédérique | Verdier, Julien | Demeret, Sophie | Berrih-Aknin, Sonia | Le Panse, Rozen

Edité par CCSD -

International audience. Circulating monocytes are mononuclear cells which are highly specialized in phagocytosis and antigen presentation, secrete a large range of different cytokines, and migrate to the tissues in response to infection and injury. Monocytes can be subdivided into three major subsets: the classical monocytes (CD14+CD16-), the intermediate monocytes (CD14+CD16+) and the non-classical monocytes (CD14-CD16+). It is suggested that classical monocytes differentiate successively into intermediate and non-classical monocytes. We investigated by flow cytometry the proportion and function of circulating monocytes in MG Patients (13 AChR+ MG patients and 10 controls). Gating on the monocyte population, we clearly observed in MG patients a significant decrease of non-classical monocytes defined as CD14-CD16+. This decrease of non-conventional monocytes was further confirmed by gating on CD14-/lowCD16+ CCR2- CD116- to exclude a potential bias due to CD16+ dendritic cells. We also analyzed serum levels in 24 AChR+ MG patients and 16 controls for cytokines known to reflect monocyte activation (sCD14 and sCD163) or cytokines involved in monocyte differentiation, such as GM-CSF, M-CSF, IL-13, IFN-γ. We did not observe any variation in serum levels of sCD14 and sCD163 in MG patients but we observed an imbalance in cytokine cocktail that might influence monocyte differentiation.Altogether, we showed a decrease of non-classical monocytes in AChR+ MG patients. This decrease might be associated with the pathophysiology of MG and need to be further investigated.

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