Synchrotron‐Based Nano‐X‐Ray Absorption Near‐Edge Structure Revealing Intracellular Heterogeneity of Iron Species in Magnetotactic Bacteria

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Chevrier, Daniel, M. | Cerdá-Doñate, Elisa | Park, Yeseul | Cacho-Nerin, Fernando | Gomez-Gonzalez, Miguel | Uebe, René | Faivre, Damien

Edité par CCSD ; Wiley -

International audience. Magnetotactic bacteria (MTB) sequester iron from the environment to biomi-neralize magnetite or greigite nanoparticles in magnetosome organelles, thoughthe necessity of intracellular iron storage for the formation process is still inquestion. Understanding the role of iron storage would make clear the contri-bution of MTB in geochemical iron cycling and its potential importance duringthe biosynthesis of application-relevant magnetic nanoparticles. Herein, howscanning X-rayfluorescence microscopy (SXFM) and nanoscale X-ray absorptionnear-edge structure (nano-XANES) mapping can spatially and chemically identifyintracellular iron species is reported, creating an opportunity to examine the roleof iron storage in magnetite biomineralization at the single-cell level. Fe K-edgenano-XANES measurements ofMagnetospirillum gryphiswaldensein varied ironmedia conditions and iron storage capacity reveal a significant quantity ofintracellular iron heterogeneities through a distinction between formed mag-netosomes and intracellular iron material. This intracellular iron component isfound in both early and late stages of biomineralization. The capabilities of nano-XANES in providing an experimental advantage in the multidisciplinaryfield ofbiomineralization are highlighted.

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