Fast optical recording of neuronal activity by three-dimensional custom-access serial holography

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Akemann, Walther | Wolf, Sébastien | Villette, Vincent | Mathieu, Benjamin | Tangara, Astou | Fodor, Jozsua | Ventalon, Cathie | Léger, Jean-François | Dieudonné, Stéphane | Bourdieu, Laurent

Edité par CCSD ; Nature Publishing Group -

International audience. Optical recording of neuronal activity in 3D brain circuits at cellular and millisecond resolution in-vivo is essential for probing information flow in the brain. While random-access multiphoton microscopy permits fast optical access to neuronal targets in 3D, the method is challenged by motion artefacts when recording from behaving animals. Therefore, we developed 3D custom-access serial holography (3D-CASH). Built upon a fast acousto-optic light modulator, 3D-CASH performs serial sampling at 40 kHz from neurons at freely selectable 3D locations. Motion artefacts are eliminated by targeting each neuron with a size-optimized pattern of excitation light covering the cell body and its anticipated displacement field. Spike rates inferred from GCaMP6f recordings in visual cortex of awake mice tracked the phase of a moving bar stimulus with higher spike correlation between intra-compared to inter-laminar neuron pairs. 3D-CASH offers access to the millisecond correlation structure of in-vivo neuronal activity in 3D microcircuits.

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