Syracuse

Cyclin G, Transcriptional Control and Developmental Stability

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Dardalhon-Cumenal, Delphine | Dupont, Camille, A | Ribeiro, Valérie | Randsholt, Neel, B | Peronnet, Frédérique | Pouch, Juliette | Le Crom, Stéphane

Edité par CCSD -

International audience. A central challenge in developmental and evolutionary biology is to understand developmental stability, the process that buffers developmental noise. We recently reported that an individual gene, Cyclin G (CycG), encoding a cyclin involved in the regulation of transcription and in the cell cycle, plays a central role in developmental stability in Drosophila (Debat et al., 2011; Debat and Peronnet, 2013). Indeed, overexpression of CycG induces an unprecedented increase in developmental noise. Using CycG overexpression as a tool, we aim to investigate the role of CycG in transcription and at highlighting gene networks underlying developmental stability. Our data show that Cyclin G interacts with Polycomb and Trithorax factors as well as with components of the spliceosome. Furthermore, several mutations of both Polycomb/Trithorax members and components of the spliceosome increase CycG-induced developmental noise. To understand more precisely the role of Cyclin G in transcription and in developmental stability control, we develop two projects : The firts one, identify transcriptional targets of cyclin G by RNA-seq and CHIP-seq. the second one, the Cyclin G involvement in alternative splicing. ✓ Analyze alternative transcripts in CycG overexpressing flies. ✓ Test the role of genes belonging to the identified sub-network in developmental stability.

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