Afficher la couverture 'Live cell immunogold labelling of RNA polymerase II | Orlov, Igor' en grand format
/5

0 avis

Live cell immunogold labelling of RNA polymerase II

Archive ouverte

Orlov, Igor | Schertel, Andreas | Zuber, Guy | Klaholz, Bruno, P. | Drillien, Robert | Weiss, Etienne | Schultz, Patrick | Spehner, Daniele

Edité par CCSD ; Nature Publishing Group -

International audience. Labeling nuclear proteins with electron dense probes in living cells has been a major challenge due to their inability to penetrate into nuclei. We developed a lipid-based approach for delivering antibodies coupled to 0.8 nm ultrasmall gold particles into the nucleus to label RNA polymerase II. Focussed Ion Beam slicing coupled to Scanning Electron Microscopy (FIB/SEM) enabled visualization of entire cells with probe localization accuracy in the 10 nm range.

Suggestions

Du même auteur

Protein delivery system containing a nickel-immobilized polymer for multimerization of affinity-purified his-tagged proteins enhances cytosolic transfer

Archive ouverte | Postupalenko, Viktoriia | CCSD

International audience

TCF12 controls oligodendroglial cell proliferation and regulates signaling pathways conserved in gliomas

Archive ouverte | Spehner, Daniele | CCSD

International audience. Abstract Diffuse gliomas are primary brain tumors originating from the transformation of glial cells. In particular, oligodendrocyte precursor cells constitute the major tumor-amplifying popu...

Cryo-FIB-SEM as a promising tool for localizing proteins in 3D

Archive ouverte | Spehner, Daniele | CCSD

International audience

Chargement des enrichissements...