Amazonian mammal monitoring using aquatic environmental DNA

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Coutant, Opale | Richard‐hansen, Cécile | de Thoisy, Benoit | Decotte, Jean‐baptiste | Valentini, Alice | Dejean, Tony | Vigouroux, Régis | Murienne, Jérôme | Brosse, Sébastien

Edité par CCSD ; Wiley/Blackwell -

International audience. Environmental DNA (eDNA) metabarcoding has emerged as one of the most efficient method to assess aquatic species presence.While the method could in theory be used to investigate non-aquatic fauna, its development for inventorying semi-aquatic andterrestrial fauna is still at its early stages. Here we aimed at investigating the reliability of aquatic eDNA metabarcodingfor inventorying mammals in Neotropical environments, be they aquatic, semi-aquatic or terrestrial. We collected aquaticeDNA in 96 sites distributed along three Guianese watersheds and compared our inventories to expected species distributionsand field observations derived from line transect samples. Species occurrences and emblematic mammals’ richness patternswere consistent with the expected distribution of the fauna and our results revealed that aquatic eDNA metabarcoding bringsadditional data to line transect samples for diurnal non-aquatic (terrestrial and arboreal) species. eDNA also provided data onspecies not detectable in line transect surveys such as semi-aquatic, aquatic and nocturnal terrestrial and arboreal species. Whilewise application of the eDNA method to inventory mammals still needs some developments to optimize sampling efficiency, itcan now be used as a complement to traditional surveys.

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