0 avis
Obstructive Apneas in a Mouse Model of Congenital Central Hypoventilation Syndrome
Archive ouverte
Edité par CCSD ; American Thoracic Society -
International audience. Rationale: Congenital central hypoventilation syndrome (CCHS) is characterized by life-threatening sleep hypoventilation and is caused by PHOX2B gene mutations, most frequently the PHOX2B 27Ala/1 mutation, with patients requiring lifelong ventilatory support. It is unclear whether obstructive apneas are part of the syndrome. Objectives: To determine if Phox2b 27Ala/1 mice, which present the main symptoms of CCHS and die within hours after birth, also express obstructive apneas, and to investigate potential underlying mechanisms.Methods: Apneas were classified as central, obstructive, or mixed by using a novel system combining pneumotachography and laser detection of abdominal movement immediately after birth. Several respiratory nuclei involved in airway patency were examined by immunohistochemistry and electrophysiology in brainstem-spinal cord preparations.Measurements and Main Results: The median (interquartile range) of obstructive apnea frequency was 2.3 (1.5-3.3)/min in Phox2b 27Ala/1 pups versus 0.6 (0.4-1.0)/min in wild types (P , 0.0001). Obstructive apnea duration was 2.7 seconds (2.3-3.9) in Phox2b 27Ala/1 pups versus 1.7 seconds (1.1-1.9) in wild types (P , 0.0001). Central and mixed apneas presented similar significant differences. In Phox2b 27Ala/1 preparations, the hypoglossal nucleus had fewer (P , 0.05) and smaller (P , 0.01) neurons, compared with wild-type preparations. Importantly, coordination of phrenic and hypoglossal motor activities was disrupted, as evidenced by the longer and variable delay of hypoglossal activity with respect to phrenic activity onset (P , 0.001).Conclusions: The Phox2b 27Ala/1 mutation predisposed pups not only to hypoventilation and central apneas, but also to obstructive and mixed apneas, likely because of hypoglossal dysgenesis. These results thus demand attention toward obstructive events in infants with CCHS.
Consulter en ligne
Suggestions
Du même auteur
