Afficher la couverture 'Mediator is an intrinsic component of the basal RNA polymerase II machinery in vivo | Lacombe, Thierry' en grand format
/5

0 avis

Mediator is an intrinsic component of the basal RNA polymerase II machinery in vivo

Archive ouverte

Lacombe, Thierry | Poh, Siew, Lay | Barbey, Régine | Kuras, Laurent

Edité par CCSD ; Oxford University Press -

International audience. Mediator is a prominent multisubunit coactivator that functions as a bridge between gene-specific activators and the basal RNA polymerase (Pol) II initiation machinery. Here, we study the poorly documented role of Mediator in basal, or activatorindependent, transcription in vivo. We show that Mediator is still present at the promoter when the Pol II machinery is recruited in the absence of an activator, in this case through a direct fusion between a basal transcription factor and a heterologous DNA binding protein bound to the promoter. Moreover, transcription resulting from activatorindependent recruitment of the Pol II machinery is impaired by inactivation of the essential Mediator subunit Med17 due to the loss of Pol II from the promoter. Our results strongly support that Mediator is an integral component of the minimal machinery essential in vivo for stable Pol II association with the promoter.

Suggestions

Du même auteur

Mediator, known as a coactivator, can act in transcription initiation in an activator-independent manner in vivo

Archive ouverte | Jean-Jacques, Hélène | CCSD

International audience. Mediator is an evolutionarily conserved complex best known for its role as a coactivator responsible for transducing regulatory signals from DNA-bound activators to the basal RNA polymerase I...

Transcriptional plasticity through differential assembly of a multiprotein activation complex

Archive ouverte | Cormier, Laëtitia | CCSD

International audience. Cell adaptation to the environment often involves induction of complex gene expression programs under the control of specific transcriptional activators. For instance, in response to cadmium,...

Substrate-mediated remodeling of methionine transport by multiple ubiquitin-dependent mechanisms in yeast cells.

Archive ouverte | Menant, Alexandra | CCSD

Plasma membrane transport of single amino-acid methionine in yeast is shown to be mediated by at least seven different permeases whose activities are transcriptionaly and post-transcriptionaly regulated by different ubiquitin-depe...

Chargement des enrichissements...