Binding and crossing: Methods for the characterization of membrane-active peptides interactions with membranes at the molecular level

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Sachon, Emmanuelle | Walrant, Astrid | Sagan, Sandrine | Cribier, Sophie | Rodriguez, Nicolas

Edité par CCSD ; Elsevier -

International audience. Antimicrobial and cell-penetrating peptides have been the object of extensive studies for more than 60 years. Initially these two families were studied separately, and more recently parallels have been drawn. These studies have given rise to numerous methodological developments both in terms of observation techniques and membrane models. This review presents some of the most recent original and innovative developments in this field, namely droplet interface bilayers (DIBs), new fluorescence approaches, force measurements, and photolabelling.

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International audience. Fluorescence-based methods are widely used to detect crossing of peptides across model or biological membranes. For membrane-active peptides, i.e., peptides that have strong membrane tropism,...

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