A comprehensive library of fluorescent constructs of SARS‐CoV‐2 proteins and their initial characterisation in different cell types

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Miserey‐lenkei, Stéphanie | Trajkovic, Katarina | d'Ambrosio, Juan, Martín | Patel, Amanda, J | Čopič, Alenka | Mathur, Pallavi | Schauer, Kristine | Goud, Bruno | Albanèse, Véronique | Gautier, Romain | Subra, Melody | Kovacs, David | Barelli, Hélène | Antonny, Bruno

Edité par CCSD ; Wiley -

International audience. Background Information. Comprehensive libraries of plasmids for SARS-CoV-2 proteins with various tags (e.g., Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein-protein interactions between the SARS-CoV-2 virus and host proteins. Results. We present here a large library of SARS CoV-2 protein constructs fused with green and red fluorescent proteins and their initial characterisation in various human cell lines including lung epithelial cell models (A549, BEAS-2B), as well as in budding yeast. The localisation of a few SARS-CoV-2 proteins matches their proposed interactions with host proteins. These include the localisation of Nsp13 to the centrosome, Orf3a to late endosomes and Orf9b to mitochondria. Conclusions and Significance. This library should facilitate further cellular investigations, notably by imaging techniques. Additional supporting information may be found online in the Supporting Information section at the end of the article.

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