Afficher la couverture 'Chromatofocusing Purification of CD1b–Antigen Complexes and Their Analysis by Isoelectric Focusing | Garcia-Alles, Luis Fernando' en grand format
/5

0 avis

Chromatofocusing Purification of CD1b–Antigen Complexes and Their Analysis by Isoelectric Focusing

Archive ouverte

Garcia-Alles, Luis Fernando | de La Salle, Henri

Edité par CCSD ; Humana Press -

International audience. The presentation of lipid antigens to T cells is mediated by the CD1 proteins. Purified functional CD1/lipid complexes are valuable tools to investigate such immune processes. Here, we describe how these complexes can be prepared in vitro, how they can be purified by chromatofocusing and how to control their antigen-loading status by isoelectric focusing.

Consulter en ligne

Suggestions

Du même auteur

Synthesis of phosphoenol pyruvate (PEP) analogues and evaluation as inhibitors of PEP-utilizing enzymes

Archive ouverte | Garcia-Alles, Luis Fernando | CCSD

International audience. The synthesis of 10 new phosphoenolpyruvate (PEP) analogues with modifications in the phosphate and the carboxylate function is described. Included are two potential irreversible inhibitors o...

Mechanism-based Inhibition of Enzyme I of the Escherichia coli Phosphotransferase System

Archive ouverte | Garcia-Alles, Luis Fernando | CCSD

International audience. Four phosphoenolpyruvate (PEP) derivatives, carrying reactive or activable chemical functions in each of the three chemical regions of PEP, were assayed as alternative substrates of enzyme I ...

Impact of Carbon Fixation, Distribution and Storage on the Production of Farnesene and Limonene in Synechocystis PCC 6803 and Synechococcus PCC 7002

Archive ouverte | Vincent, Marine | CCSD

International audience. Terpenes are high-value chemicals which can be produced by engineered cyanobacteria from sustainable resources, solar energy, water and CO2. We previously reported that the euryhaline unicell...

Chargement des enrichissements...