YAP protects the eye from uveitic glaucoma by preserving retinal and anterior chamber homeostases

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Bitard, Juliette | Grellier, Elodie-Kim | Lourdel, Sophie | Filipe, Helena | Hamon, Annaïg | Fenaille, François | Castelli, Florence | Chu-Van, Emeline | Roger, Jérôme E. | Locker, Morgane | Perron, Muriel

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https://iovs.arvojournals.org/article.aspx?articleid=2775349. International audience. The role of the transcription coactivator YAP has been studied during eye development but its implication in ocular homeostasis and diseases is much less documented. YAP is expressed by several ocular cell types including the non-pigmented cells of the ciliary body (CB) as well as Müller glial cells (MGs) of the neuroretina. Here, we assessed whether postnatal Yap deletion in these two cell types could affect eye homeostasis during aging.Methods : Specific Yap deletion in CB and MGs was induced in a conditional mouse model (Yap cKO) at P10. The phenotype was investigated by western-blot, immunohistochemistry, and RNA-sequencing (RNA-seq). Blood defects were identified by fluorescein angiography. Optic nerve degeneration was assessed by optical coherence tomography. Intraocular pressure (IOP) was recorded by a rebound tonometer. Imaging of the CB in healthy and uveitic human patients was performed by ultrasound biomicroscopy.

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