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Passive coupling of membrane tension and cell volume during active response of cells to osmosis

Archive ouverte | Roffay, Chloé | CCSD

International audience. During osmotic changes of their environment, cells actively regulate their volume and plasma membrane tension that can passively change through osmosis. How tension and volume are coupled dur...

Technical insights into fluorescence lifetime microscopy of mechanosensitive Flipper probes

Archive ouverte | Roffay, Chloé | CCSD

Abstract Measuring forces within living cells remains a technical challenge. We developed hydrophobic mechanosensing fluorescent probes called Flippers, whose fluorescence lifetime depends on lipid packing and can report on membra...

Tutorial: fluorescence lifetime microscopy of membrane mechanosensitive Flipper probes

Archive ouverte | Roffay, Chloé | CCSD

International audience

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