Longitudinal high-resolution imaging through a flexible intravital imaging window

Archive ouverte

Jacquemin, Guillaume | Benavente-Diaz, Maria | Djaber, Samir | Bore, Aurélien | Dangles-Marie, Virginie | Surdez, Didier | Tajbakhsh, Shahragim | Fre, Silvia | Lloyd-Lewis, Bethan

Edité par CCSD ; American Association for the Advancement of Science (AAAS) -

International audience. Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present a silicone-based imaging window consisting of a fully flexible, sutureless design that is ideally suited for long-term, longitudinal IVM of growing tissues and tumors. Crucially, we show that this window, without any customization, is suitable for numerous anatomical locations in mice using a rapid and standardized implantation procedure. This low-cost device represents a substantial technological and performance advance that facilitates intravital imaging in diverse contexts in higher organisms, opening previously unattainable avenues for in vivo imaging of soft and fragile tissues.

• Description
• Sujet/Public
• Outil
• Outil d'anticipation
• Mémoire et thèse
• Gestion