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RocS drives chromosome segregation and nucleoid protection in Streptococcus pneumoniae
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Edité par CCSD ; Nature Publishing Group -
Work by the Grangeasse lab is supported by grants from the CNRS, the University of Lyon, the Agence National de la Recherche (ANR-10-BLAN-1303-01 and ANR-15-CE32-0001-01), the Region Auvergne-Rhone-Alpes (financial support for C.M. and P.S.G.), the 'Fondation pour la Recherche Medicale' (financial support for N.D. (ING20150532637) and C.M. (FDT20170437272)) and the Bettencourt-Schueller Foundation. Work by the Veening lab is supported by the Swiss National Science Foundation (project grant 31003A_172861), a JPIAMR grant (50-52900-98-202) from the Netherlands Organization for Health Research and Development (ZonMW) and the ERC consolidator grant 771534-PneumoCaTChER. We thank S. Ravaud for help in RocS structural predictions, A. Fenton (University of Sheffield, Sheffield, UK) for providing us with the D39 Delta cps strain and K. Weaver (University of South Dakota, Vermillion, SD, USA) for providing the pAD1 plasmid. We acknowledge the contribution of the Protein Science of the SFR Biosciences Gerland-Lyon Sud (UMS344/US8).
Work by the Grangeasse lab is supported by grants from the CNRS, the University of Lyon, the Agence National de la Recherche (ANR-10-BLAN-1303-01 and ANR-15-CE32-0001-01), the Region Auvergne-Rhone-Alpes (financial support for C.M. and P.S.G.), the 'Fondation pour la Recherche Medicale' (financial support for N.D. (ING20150532637) and C.M. (FDT20170437272)) and the Bettencourt-Schueller Foundation. Work by the Veening lab is supported by the Swiss National Science Foundation (project grant 31003A_172861), a JPIAMR grant (50-52900-98-202) from the Netherlands Organization for Health Research and Development (ZonMW) and the ERC consolidator grant 771534-PneumoCaTChER. We thank S. Ravaud for help in RocS structural predictions, A. Fenton (University of Sheffield, Sheffield, UK) for providing us with the D39 Delta cps strain and K. Weaver (University of South Dakota, Vermillion, SD, USA) for providing the pAD1 plasmid. We acknowledge the contribution of the Protein Science of the SFR Biosciences Gerland-Lyon Sud (UMS344/US8).. International audience.
Chromosome segregation in bacteria is poorly understood outside some prominent model strains(1-5) and even less is known about how it is coordinated with other cellular processes. This is the case for the opportunistic human pathogen Streptococcus pneumoniae (the pneumococcus)(6), which lacks the Min and the nucleoid occlusion systems(7), and possesses only an incomplete chromosome partitioning Par(A)BS system, in which ParA is absent(8). The bacterial tyrosine kinase(9) CpsD, which is required for capsule production, was previously found to interfere with chromosome segregation(10). Here, we identify a protein of unknown function that interacts with CpsD and drives chromosome segregation. RocS (Regulator of Chromosome Segregation) is a membrane-bound protein that interacts with both DNA and the chromosome partitioning protein ParB to properly segregate the origin of replication region to new daughter cells. In addition, we show that RocS interacts with the cell division protein FtsZ and hinders cell division. Altogether, this work reveals that RocS is the cornerstone of a nucleoid protection system ensuring proper chromosome segregation and cell division in coordination with the biogenesis of the protective capsular layer.
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