H2O2 and Engrailed 2 paracrine activity synergize to shape the zebrafish optic tectum

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Amblard, Irène | Thauvin, Marion | Rampon, Christine | Queguiner, Isabelle | Pak, Valeriy, V | Belousov, Vsevolod | Prochiantz, Alain | Volovitch, Michel | Joliot, Alain | Vriz, Sophie

Edité par CCSD ; Nature Publishing Group -

International audience. Although a physiological role for redox signaling is now clearly established, the processes sensitive to redox signaling remains to be identified. Ratiometric probes selective for H 2 O 2 have revealed its complex spatiotemporal dynamics during neural development and adult regeneration and perturbations of H 2 O 2 levels disturb cell plasticity and morphogenesis. Here we ask whether endogenous H 2 O 2 could participate in the patterning of the embryo. We find that perturbations of endogenous H 2 O 2 levels impact on the distribution of the Engrailed homeoprotein, a strong determinant of midbrain patterning. Engrailed 2 is secreted from cells with high H 2 O 2 levels and taken up by cells with low H 2 O 2 levels where it leads to increased H 2 O 2 production, steering the directional spread of the Engrailed gradient. These results illustrate the interplay between protein signaling pathways and metabolic processes during morphogenetic events.

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