A simple ATAC-seq protocol for population epigenetics

Archive ouverte

Augusto, Ronaldo de Carvalho | REY, Olivier | Cosseau, Céline | Chaparro, Cristian | Vidal-Dupiol, Jeremie | Allienne, Jean-François | Duval, David | Pinaud, Silvain | Tönges, Sina | Andriantsoa, Ranja | Luquet, Émilien | Aubret, Fabien | Dia Sow, Mamadou | David, Patrice | Thomson, Vicki | Joly, Dominique, D. | Lima, Mariana Gomes | Federico, Déborah | Danchin, Etienne | Minoda, Aki | Grunau, Christoph

Edité par CCSD ; F1000Research -

version 2; peer review: 2 approved. International audience. We describe here a protocol for the generation of sequence-ready libraries for population epigenomics studies, and the analysis of alignment results. We show that the protocol can be used to monitor chromatin structure changes in populations when exposed to environmental cues. The protocol is a streamlined version of the Assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) that provides a positive display of accessible, presumably euchromatic regions. The protocol is straightforward and can be used with small individuals such as daphnia and schistosome worms, and probably many other biological samples of comparable size (~10,000 cells), and it requires little molecular biology handling expertise.

• Description
• Sujet/Public
• Outil
• Outil d'anticipation
• Mémoire et thèse
• Gestion