TgAP2IX-5 is a key transcriptional regulator of the asexual cell cycle division in Toxoplasma gondii

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Khelifa, Asma, S. | Guillen Sanchez, Cecilia | Lesage, Kevin, M. | Huot, Ludovic | Mouveaux, Thomas | Péricard, Pierre | Barois, Nicolas | Touzet, Hélène | Marot, Guillemette | Roger, Emmanuel | Gissot, Mathieu

Edité par CCSD ; Nature Publishing Group -

International audience. Apicomplexan parasites have evolved efficient and distinctive strategies for intracellular replication where the timing of emergence of the daughter cells (budding) is a decisive element. However, the molecular mechanisms that provide the proper timing of parasite budding remain unknown. Using Toxoplasma gondii as a model Apicomplexan, we identified a master regulator that controls the timing of the budding process. We show that an ApiAP2 transcription factor, TgAP2IX-5, controls cell cycle events downstream of centrosome duplication. TgAP2IX-5 binds to the promoter of hundreds of genes and controls the activation of the budding-specific cell cycle expression program. TgAP2IX-5 regulates the expression of specific transcription factors that are necessary for the completion of the budding cycle. Moreover, TgAP2IX-5 acts as a limiting factor that ensures that asexual proliferation continues by promoting the inhibition of the differentiation pathway. Therefore, TgAP2IX-5 is a master regulator that controls both cell cycle and developmental pathways.

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