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A fast, efficient and easy to implement method to purify bacterial pili from Lacticaseibacillus rhamnosus GG based on multimodal chromatography

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dos Santos Morais, Raphael | El-Kirat-Chatel, Sofiane | Burgain, Jennifer | Simard, Blandine | Barrau, Sarah | Paris, Cedric | Borges, Frédéric | Gaiani, Claire

Edité par CCSD ; Frontiers Media -

International audience. Pili are polymeric proteins located at the cell surface of bacteria. These filamentous proteins play a pivotal role in bacterial adhesion with the surrounding environment. They are found both in Gram-negative and Gram-positive bacteria but differ in their structural organization. Purifying these high molecular weight proteins is challenging and has certainly slowed down

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