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Outcomes of honeybee pupae inoculated with deformed wing virus genotypes A and B

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Dubois, Eric | Dardouri, Marine | Schurr, Frank | Cougoule, Nicolas | Sircoulomb, Fabrice | Thiéry, Richard

Edité par CCSD ; Springer Verlag -

International audience. Deformed wing virus and Varroa destructor virus-1 have a high percentage of nucleotide identity and might be considered as closely related viruses: DWV genotype A (DWV-A) and DWV genotype B (DWV-B) respectively. They have been implicated in overwinter colony losses in association with Varroa destructor infestations that vectored both DWV variants. In this study, we performed experimental inoculations of honeybee pupae with viral suspensions prepared from honeybee heads naturally infected by either DWV-A or DWV-B. Two outcomes were observed: the inoculated pupae exhibited either higher rates of bees with deformed wings or higher mortality rates than control bees. For both DWV variants, the viral loads quantified in the head of inoculated bees were significantly greater than those in control bees (p < 0.01). These outcomes were not correlated to the virus genotype (DWV-A or DWV-B) detected in the inocula by RT-quantitative PCR targeting the VP3 coding sequence (RT-qPCR). However, the highest mortality rates found in our study were correlated with an increase in sacbrood virus (SBV) load. Despite the fact that only either DWV-A or DWV-B was expected to be inoculated, we observed increased mortality in honeybees that were infected with both DWV and SBV.

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