0 avis
A Spatiotemporal Sequence of Sensitization to Slits and Semaphorins Orchestrates Commissural Axon Navigation
Archive ouverte
Edité par CCSD ; Elsevier Inc -
International audience. Highlights d Live monitoring of guidance receptors reveals specific dynamics in commissural axons d Slit and Semaphorin receptors have different temporal patterns of membrane insertion d A front-rear polarity organizes PlxnA1 and Robo1 receptors at the growth-cone surface d Robo1, but not Robo2, is sorted during midline crossingAccurate perception of guidance cues is crucial for cell and axon migration. During initial navigation in the spinal cord, commissural axons are kept insensitive to midline repellents. Upon midline crossing in the floor plate, they switch on responsiveness to Slit and Semaphorin repulsive signals and are thus propelled away and prevented from crossing back. Whether and how the different midline repellents control specific aspects of this navigation remain to be elucidated. We set up a paradigm for live-imaging and super-resolution analysis of PlexinA1, Neuropilin-2, and Robo1/2 receptor dynamics during commissural growth cone navigation in chick and mouse embryos. We uncovered a remarkable program of sensitization to midline cues achieved by unique spatiotemporal sequences of receptor allocation at the growth-cone surface that orchestrates receptor-specific growth-cone behavior changes. This reveals post-translational mechanisms whereby coincident guidance signals are temporally resolved to allow the generation of specific guidance responses.
Consulter en ligne
Suggestions
Du même auteur
