Genetics of nodulation in Aeschynomene evenia uncovers new mechanisms of the rhizobium-legume symbiosis

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Quilbé, Johan | Lamy, Léo | Brottier, Laurent | Leleux, Philippe | Fardoux, Joel | Rivallan, Ronan | Benichou, Thomas | Guyonnet, Rémi | Becana, Manuel | Villar, Irene | Garsmeur, Olivier | Hufnagel, Bárbara | Delteil, Amandine | Gully, Djamel | Chaintreuil, Clémence | Pervent, Marjorie | Cartieaux, Fabienne | Bourge, Mickaël | Valentin, Nicolas | Martin, Guillaume | Fontaine, Loïc | Droc, Gaëtan | Dereeper, Alexis | Farmer, Andrew | Libourel, Cyril | Nouwen, Nico | Gressent, Frederic | Mournet, Pierre | D’hont, Angélique | Giraud, Eric | Klopp, Christophe | Arrighi, Jean-François

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Among legumes (Fabaceae) capable of nitrogen-fixing nodulation, several Aeschynomene spp. use a unique symbiotic process that is independent of Nod factors and infection threads. They are also distinctive in developing root and stem nodules with photosynthetic bradyrhizobia. Despite the significance of these symbiotic features, their understanding remains limited. To overcome such limitations, we conducted genetic studies of nodulation in Aeschynomene evenia , supported by the development of a genome sequence for A. evenia and transcriptomic resources for 10 additional Aeschynomene spp. Comparative analysis of symbiotic genes substantiated singular mechanisms in the early and late nodulation steps. A forward genetic screen also showed that AeCRK , coding a novel receptor-like kinase, and the symbiotic signaling genes AePOLLUX, AeCCamK, AeCYCLOPS, AeNSP2 and AeNIN , are required to trigger both root and stem nodulation. This work demonstrates the utility of the A. evenia model and provides a cornerstone to unravel new mechanisms underlying the rhizobium-legume symbiosis.

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