Improving the antioxidant properties of Calophyllum inophyllum L. seed oil from French Polynesia: development and biological applications of ethanol-soluble resin extracts

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Cassien, Mathieu | Mercier, Anne | Thétiot-Laurent, Sophie | Culcasi, Marcel | Asteian, Alice | Ricquebourg, Emilie | Herbette, Gaëtan | Bianchini, Jean-Pierre | Raharivelomanana, Phila | Pietri, Sylvia

Edité par CCSD -

Tamanu oil (TO) from Calophyllum inophyllum L. has long been used for its numerous properties in traditional medicine. With the aim to investigate the intrinsic antioxidant and pharmacological activities of the complex pool of constituents contained in the resin part of TO, extraction with methanol and ethanol were undertaken to give MeTO and EtTO extracts, this latter being further partitioned into neutral (NTR) and acidic (ATR) fractions. Further LPLC/HPLC separations led to sixteen pure metabolites. For two of them, calanolide D and 12-oxocalanolide A, we report here their first identification from a natural source. Extracts, subfractions and metabolites were evaluated for their antioxidant, anti-inflammatory, antimicrobial (Staphylococcus aureus) and antimycobacterial (Mycobacterium tuberculosis H37RV) properties, and their protective effect on tBuOOH-exposed cells and UV-exposed rat skin slices. Data showed that NTR and ATR resin fractions gathering the main amounts of bioactive compounds exhibited enhanced antioxidant and anti-inflammatory activities as compared to the lipid phase of TO. Antimicrobial activity was highlighted for NTR, while ATR was more cytoprotective in cells and rat tissues. All together, data suggest that ethanol-soluble fractions enriched in phenolics, pyranocoumarin derivatives and flavonoids could be of interest for further development of new and better bioavailable medicinal preparation.

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