X-ray and UV radiation-damage-induced phasing using synchrotron serial crystallography

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Foos, Nicolas | Seuring, Carolin | Schubert, Robin | Burkhardt, Anja | Svensson, Olof | Meents, Alke | Chapman, Henry N. | Nanao, Max H.

Edité par CCSD ; International Union of Crystallography -

International audience. Specific radiation damage can be used to determine phases de novo from macromolecular crystals. This method is known as radiation-damage-induced phasing (RIP). One limitation of the method is that the dose of individual data sets must be minimized, which in turn leads to data sets with low multiplicity. A solution to this problem is to use data from multiple crystals. However, the resulting signal can be degraded by a lack of isomorphism between crystals. Here, it is shown that serial synchrotron crystallography in combination with selective merging of data sets can be used to determine high-quality phases for insulin and thaumatin, and that the increased multiplicity can greatly enhance the success rate of the experiment.

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