SARS-CoV-2 targets neurons of 3D human brain organoids

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Ramani, Anand | Müller, Lisa | Ostermann, Philipp, N | Gabriel, Elke | Abida-Islam, Pranty | Müller-Schiffmann, Andreas | Mariappan, Aruljothi | Goureau, Olivier | Gruell, Henning | Walker, Andreas | Andrée, Marcel | Hauka, Sandra | Houwaart, Torsten | Dilthey, Alexander | Wohlgemuth, Kai | Omran, Heymut | Klein, Florian | Wieczorek, Dagmar | Adams, Ortwin | Timm, Jörg | Korth, Carsten | Schaal, Heiner | Gopalakrishnan, Jay

Edité par CCSD ; EMBO Press -

International audience. COVID-19 pandemic caused by SARS-CoV-2 infection is a public health emergency. COVID-19 typically exhibits respiratory illness. Unexpectedly, emerging clinical reports indicate that neurological symptoms continue to rise, suggesting detrimental effects of SARS-CoV-2 on the central nervous system (CNS). Here, we show that a Düsseldorf isolate of SARS-CoV-2 enters 3D human brain organoids within 2 days of exposure. We identified that SARS-CoV-2 preferably targets neurons of brain organoids. Imaging neurons of organoids reveal that SARS-CoV-2 exposure is associated with altered distribution of Tau from axons to soma, hyperphosphorylation, and apparent neuronal death. Our studies, therefore, provide initial insights into the potential neurotoxic effect of SARS-CoV-2 and emphasize that brain organoids could model CNS pathologies of COVID-19.

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