Distinct genetic bases for plant root responses to lipo-chitooligosaccharide signal molecules from distinct microbial origins

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Bonhomme, Maxime | Bensmihen, Sandra | André, Olivier | Amblard, Emilie | Garcia, Magali | Maillet, Fabienne | Puech Pagès, Virginie | Gough, Clare | Fort, Sebastien | Cottaz, Sylvain | Bécard, Guillaume | Jacquet, Christophe

Edité par CCSD ; Oxford University Press (OUP) -

International audience. Lipo-chitooligosaccharides (LCOs) were originally found as symbiotic signals called Nod Factors (Nod-LCOs) controlling nodulation of legumes by rhizobia. More recently LCOs were also found in symbiotic fungi and, more surprisingly, very widely in the kingdom fungi including in saprophytic and pathogenic fungi. The LCO-V(C18:1, Fuc/MeFuc), hereafter called Fung-LCOs, are the LCO structures most commonly found in fungi. This raises the question of how legume plants, such as Medicago truncatula, can discriminate between Nod-LCOs and these Fung-LCOs. To address this question, we performed a Genome Wide Association Study on 173 natural accessions of Medicago truncatula, using a root branching phenotype and a newly developed local score approach. Both Nod-and Fung-LCOs stimulated root branching in most accessions but the root responses to these two types of LCO molecules were not correlated. Also, heritability of root response was higher for Nod-LCOs than for Fung-LCOs. We identified 123 loci for Nod-LCO and 71 for Fung-LCO responses, but only one was common. This suggests that Nod-and Fung-LCOs both control root branching but use different molecular mechanisms. The tighter genetic constraint of the root response to Fung-LCOs possibly reflects the ancestral origin of the biological activity of these molecules.

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