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Isolation and characterization of microsatellite loci in the Norway lobster (Nephrops norvegicus)

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Streiff, Réjane, D | Guillemaud, Thomas | Alberto, Filipe | Magalhaes, J | Castro, Margarida | Cancela, M. Leonor

Edité par CCSD ; Wiley-Blackwell -

International audience. To assess the level and spatial pattern of genetic diversity of a marine crustacean species, Nephrops norvegicus , hypervariable molecular markers were obtained by screening of a partial genomic library enriched for microsatellite dinucleotide motifs. Following sequen-cing of the positive clones, 10 pairs of primers were designed and five of them were shown to detect polymorphic loci. These loci were checked for their variability in two natural populations , fished off the Portuguese coast. They showed a high variability for the number of alleles, and for the expected and observed heterozygosities. No significant differentiation between the two localities was observed.

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