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Label-free 1-DE-LC-MS/MS to identify arbuscular mycorrhiza-related membrane proteins

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Abdallah, Cosette, C. | Renaut, Jenny | Valot, Benoît | Zivy, Michel | Recorbet, Ghislaine | Wipf, Daniel, D. | Dumas-Gaudot, Eliane

Edité par CCSD -

SPE IPM. National audience. Deep changes in the shape and number of organelles, together with profound modifications in various membrane compartments, are induced within arbuscular mycorrhizal (AM) symbiosis. In this context, to investigate the membrane-associated proteins that are regulated in the model interaction Medicago truncatula – Rhizophagus irregularis, label-free 1DE-LC-MS/MS approach has been employed as alternative to two-dimensional gel electrophoresis. The existence of a correlation between protein abundance and peak areas or number of MS/MS spectra has widened the choice of label-free quantitative proteomics. The results highlighted microsomal protein candidates that could be involved in the symbiotic exchanges between plant and fungal cells.

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