Syracuse

Development of high-cell-density fermentation for heterologous interleukin 1beta production in Kluyveromyces lactis controlled by the PH05 promoter

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Blondeau, K. | Boutur, O. | Boze, Hélène | Jung, G. | Moulin, Gabrielle | Galzy, P.

Edité par CCSD ; Springer Verlag -

International audience. The use of a phosphate organic form taken up by Kluyveromyces lactis removes repression of the PHO5 promoter and releases heterologous interleukin 1 synthesis while providing sufficient phosphate for growth. The oxidative metabolism of high-cell-density fed-batch and chemostat cultures was thus maintained under derepressed protein synthesis conditions. Interleukin 1 production was then growth-associated, an unusual mode of protein synthesis regulation under the control of the PHO5 promoter.

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