Isolation of cinnamyl alcohol dehydrogenase cDNAs from two important economic species : alfalfa and poplar. Demonstration of a high homology of the gene within angiosperms

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van Doorsselaere, J. | Baucher, Marie | Feuillet, Catherine | Boudet, A.M. | van Montagu, Marc | Inzé, Dirk

Edité par CCSD ; Elsevier -

International audience. Cinnamyl alcohol dehydrogenase (CAD), a key enzyme in lignification, was cloned from poplar (Populus trichocarpa x P. deltoides) and alfalfa (Medicago sativa L.). Both cDNAs contain one continuous open reading frame encoding a protein of 357 amino acid residues (M(r) 39,036) for the poplar CAD and 358 amino acids (M(r) 38,950) for the alfalfa CAD. Comparison of the poplar and alfalfa CAD with other reported plant CAD sequences confirms the identity of the isolated cDNAs and shows the poplar and alfalfa CAD to be closely related to CAD from tobacco, eucalyptus and Aralia cordata (80% amino acid identity) while 70% amino acid identity was found with the CAD from the gymnosperm spruce. Sequence comparisons allowed also to identify important conserved domains and specific functional motifs.

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