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Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains

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López-Goñi, I. | García-Yoldi, D. | Marin, C.M. | de Miguel, M.J. | Muñoz, P.M. | Blasco, J.M. | Jacques, Isabelle | Grayon, Maggy | Cloeckaert, Axel | Ferreira, A.C. | Cardoso, R. | de Sá, M. I. Corrêa | Walravens, K. | Albert, D. | Garin-Bastuji, B.

Edité par CCSD ; American Society for Microbiology -

International audience. An evaluation of a multiplex PCR assay (Bruce-ladder) was performed in seven laboratories using 625 Brucella strains from different animal and geographical origins. This robust test can differentiate in a single step all of the classical Brucella species, including those found in marine mammals and the S19, RB51, and Rev.1 vaccine strains.

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