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Characterization of microsatellite markers in mandarin orange (Citrus reticulata Blanco)

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Froelicher, Yann | Dambier, Dominique | Bassene, Jean-Baptiste | Costantino, Gilles | Lotfy, Samir | Didout, Christophe | Beaumont, Vincent | Brottier, Philippe | Risterucci, Ange-Marie | François, Luro | Ollitrault, Patrick

Edité par CCSD ; Wiley/Blackwell -

International audience. A dinucleotide-enriched genomic library was obtained from mandarin orange (Citrus reticulata Blanco). A subset of 101 positive clones was sequenced and primers were designed. The loci were screened for levels of variation using 26-29 wild mandarin oranges collected in Vietnam. Forty-three loci were polymorphic with the number of alleles ranging from two to 18. The observed heterozygosity (H-O) and expected heterozygosity (H-E) were from 0.03 to 0.96 and from 0.03 to 0.92, respectively.

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