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Genome Sequence of Bartonella birtlesii, a Bacterium Isolated from Small Rodents of the Genus Apodemus
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Edité par CCSD ; American Society for Microbiology -
International audience. Bacteria of the genus Bartonella are facultative intracellular bacteriathat use arthropod transmission and invasion of redblood cells as a parasitismstrategy (8).Bartonella species belong tothe -2 subgroup of Proteobacteria and are fastidious Gram-negativebacteria highly adapted to their mammalian reservoir hosts,where they live within erythrocytes. To date, more than 30 Bartonellaspecies have been isolated from mammals (including humansand domestic and wild animals), and half of them are consideredemerging pathogens (6). Bartonella birtlesii has beenisolated for the first time fromthe blood of smallmammals of thegenusApodemus in France and theUnitedKingdom(1).Althoughits pathogenicity in humans is unknown, this bacterium has beenmainly used as a model to study long-termbacteremia and erythrocyteadhesion and invasion in mice (2, 9). Using signaturetaggedmutagenesis of this bacterium, components of the type IVsecretion system (T4SS) Trw were identified as the molecular determinantfor host-specific erythrocyte infection (9). Therefore, inorder to be able to identify any disrupted genes in this signaturetaggedmutagenesis library, we sequenced the genome of B.birtlesii.Genomic DNA fromB. birtlesii (strain IBS 325T, CIP 106294T)was fully sequenced by pyrosequencing using a Titaniumgenomesequencer (454 Life Sciences, Branford, CT) (5). A library ofpaired-end fragments was created following the manufacturer’sinstructions (454 Life Sciences). This library was sequenced usinga GS Titanium sequencer (454 Life Sciences) (5).Reads originated from the strain were assembled into contigs,and a scaffold was created using Newbler 2.53 (454 Life Sciences,Branford, CT). The assembly was verified using CLC Genomicssoftware (CLC Bio, MA). Open reading frame (ORF) predictionwas performed on the wild-type strain using PRODIGAL (3). Predictedproteins were compared against the GenBank nonredundantdatabase using BLASTP (http://www.ncbi.nlm.nih.gov) forfunctional annotation. tRNAs and rRNAs were predicted usingARAGORN (4) and RNAMMER (3), respectively.B. birtlesii draft genome sequencing generated one scaffoldcontaining 35 contigs with an average coverage of 27-fold. Thegenome exhibits a total size of 1,833,875 bp and a G C content of37%. A total of 1,648 protein-coding ORFs with an average size of871 bp were identified and cover 78.6% of the genome. Amongthose genes we found 106 ORFans (6.4% of the total gene content),42 tRNAs, and two ribosomal operons with the 3 expectedRNA genes (5S, 23S, and 16S). Other notable functional featureswere two type IV secretion system operons (VirB and Trw). Weidentified 1,250 orthologous genes between B. birtlesii and Bartonellatribocorum, themost closely relatedBartonella species whosegenome has been sequenced (7), using a reciprocal best BLAST hitstrategy.Nucleotide sequence accession numbers. The results fromthis whole-genome shotgun project have been depositedwith DDBJ/EMBL/GenBank under the accession numberAKIP00000000. The version described in this paper is the firstversion, AKIP01000000.
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