Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies

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Breard, Emmanuel | Lara, Estelle | Comtet, Loic | Viarouge, Cyril | Doceul, Virginie | Desprat, Alexandra | Vitour, Damien | Pozzi, Nathalie | Cay, Ann Brigitte | de Regge, Nick | Pourquier, Philippe | Schirrmeier, Horst | Hoffmann, Bernd | Beer, Martin | Sailleau, Corinne | Zientara, Stéphan

Edité par CCSD ; Public Library of Science -

International audience. A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derived from sheep, goat and bovine sera collected in France and Germany (n = 1515) in 2011 and 2012 were categorized according to the results of a virus neutralization test (VNT) or an indirect immuno-fluorescence assay (IFA). The specificity was evaluated with 1364 sera from sheep, goat and bovine collected in France and Belgium before 2009. Overall agreement between VNT and ELISA was 98.9% and 98.3% between VNT and IFA, indicating a very good concordance between the different techniques. Although cross-reactions with other Orthobunyavirus from the Simbu serogroup viruses might occur, it is a highly sensitive, specific and robust ELISA-test validated to detect anti-SBV antibodies. This test can be applied for SBV sero-diagnostics and disease-surveillance studies in ruminant species in Europe.

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