High-resolution melt PCR analysis for rapid identification of emChlamydia abortus/em live vaccine strain 1B among emC. abortus/em strains and field isolates

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High-resolution melt PCR analysis for rapid identification of Chlamydia abortus live vaccine strain 1B among C. abortus strains and field isolates

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Edité par CCSD ; Elsevier -

International audience. We describe a novel high-resolution melt assay that clearly differentiates Chlamydia abortus live vaccine strain 1B from field C. abortus strains and field wild-type isolates based on previously described single nucleotide polymorphisms. This modern genotyping technique is inexpensive, easy to use, and less time-consuming than PCR-RFLP.

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