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A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants

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Larrieu, Antoine | Champion, Antony | Legrand, Jonathan | Lavenus, Julien | Mast, David | Brunoud, Geraldine | Oh, Jaesung | Guyomarc'H, Soazig | Pizot, Maxime | Farmer, Edward E. | Turnbull, Colin | Vernoux, Teva | Bennett, Malcolm J. | Laplaze, Laurent

Edité par CCSD ; Nature Publishing Group -

International audience. Activated forms of jasmonic acid (JA) are central signals coordinating plant responses to stresses, yet tools to analyse their spatial and temporal distribution are lacking. Here we describe a JA perception biosensor termed Jas9-VENUS that allows the quantification of dynamic changes in JA distribution in response to stress with high spatiotemporal sensitivity. We show that Jas9-VENUS abundance is dependent on bioactive JA isoforms, the COI1 co-receptor, a functional Jas motif and proteasome activity. We demonstrate the utility of Jas9-VENUS to analyse responses to JA in planta at a cellular scale, both quantitatively and dynamically. This included using Jas9-VENUS to determine the cotyledon-to-root JA signal velocities on wounding, revealing two distinct phases of JA activity in the root. Our results demonstrate the value of developing quantitative sensors such as Jas9-VENUS to provide high-resolution spatiotemporal data about hormone distribution in response to plant abiotic and biotic stresses.

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