Afficher la couverture 'Identification and characterization of an ABA-activated MAP kinase cascade in <em>Arabidopsis thaliana</em> | Danquah, Agyemang' en grand format
/5

0 avis

Identification and characterization of an ABA-activated MAP kinase cascade in Arabidopsis thaliana

Archive ouverte

Danquah, Agyemang | de Zélicourt, Axel | Boudsocq, Marie | Neubauer, Jorinde | Frei Dit Frey, Nicolas | Leonhardt, Nathalie | Pateyron, Stephanie | Gwinner, Frederik | Tamby, Jean-Philippe | Ortiz-Masia, Dolores | Marcote, Maria J. | Hirt, Heribert | Colcombet, Jean

Edité par CCSD ; Wiley -

International audience. Abscisic acid (ABA) is a major phytohormone involved in important stress-related and developmental plant processes. Recent phosphoproteomic analyses revealed a large set of ABA-triggered phosphoproteins as putative mitogen-activated protein kinase (MAPK) targets, although the evidence for MAPKs involved in ABA signalling is still scarce. Here, we identified and reconstituted in vivo a complete ABA-activated MAPK cascade, composed of the MAP3Ks MAP3K17/18, the MAP2K MKK3 and the four C group MAPKs MPK1/2/7/14. In planta, we show that ABA activation of MPK7 is blocked in mkk3-1 and map3k17mapk3k18 plants. Coherently, both mutants exhibit hypersensitivity to ABA and altered expression of a set of ABA-dependent genes. A genetic analysis further reveals that this MAPK cascade is activated by the PYR/PYL/RCAR-SnRK2-PP2C ABA core signalling module through protein synthesis of the MAP3Ks, unveiling an atypical mechanism for MAPK activation in eukaryotes. Our work provides evidence for a role of an ABA-induced MAPK pathway in plant stress signalling.

Consulter en ligne

Suggestions

Du même auteur

Plant MAPK cascades: just rapid signaling modules?

Archive ouverte | Boudsocq, Marie | CCSD

International audience. Abscisic acid (ABA) is a major phytohormone mediating important stress-related processes. We recently unveiled an ABA-activated MAPK signaling module constituted of MAP3K17/18-MKK3-MPK1/2/7/1...

The role of ABA and MAPK signaling pathways in plant abiotic stress responses

Archive ouverte | Danquah, Agyemang | CCSD

International audience. As sessile organisms, plants have developed specific mechanisms that allow them to rapidly perceive and respond to stresses in the environment. Among the evolutionarily conserved pathways, th...

Constitutively Active Mitogen-Activated Protein Kinase Versions Reveal Functions of Arabidopsis MPK4 in Pathogen Defense Signaling

Archive ouverte | Berriri, Souha | CCSD

International audience. Plant mitogen-activated protein kinases (MAPKs) are involved in important processes, including stress signaling and development. In a functional yeast screen, we identified mutations that ren...

Chargement des enrichissements...