High-throughput sequencing of complete genomes of ipomoviruses associated with an epidemic of cassava brown streak disease in the Comoros Archipelago

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Scussel, Sarah | Candresse, Thierry | Marais, Armelle | Claverie, Sohini | Hoareau, Murielle | Azali, Hamza A. | Verdin, Eric | Tepfer, Mark | Filloux, Denis | Fernandez, Emmanuel | Roumagnac, Philippe | Robène, Isabelle | Lefeuvre, Pierre | Jourda, Cyril | Roux-Cuvelier, Michel | Lett, Jean-Michel

Edité par CCSD ; Springer Verlag -

International audience. Using high-throughput sequencing of small interfering RNAs (siRNAs), virion-associated nucleic acid (VANA), and double stranded RNAs (dsRNAs), we have determined the complete genome sequences of Comorian isolates of two ipomoviruses, cassava brown streak virus (CBSV) and a divergent isolate of Ugandan cassava brown streak virus (UCBSV-KM) representing a new strain of this virus. While the large ORF of CBSV shares the highest nucleotide sequence identity (95.9%) with a Tanzanian isolate of CBSV, the large UCBSV-KM ORF shares the highest nucleotide sequence identity (77.5%) with a Malawian isolate of UCBSV. This low value is near the species demarcation threshold for the family Potyviridae (<76%). Phylogenetic analysis confirms that UCBSV-KM represents a new lineage that is genetically distinct from the currently described UCBSV strains.

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