Novel circular DNA viruses associated with Apiaceae and Poaceae from South Africa and New Zealand

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Richet, Cecile | Kraberger, Simona | Filloux, Denis | Bernardo, Pauline | Harkins, Gordon W. | Martin, Darren P. | Roumagnac, Philippe | Varsani, Arvind

Edité par CCSD ; Springer Verlag -

International audience. Advances in molecular techniques used in viral metagenomics coupled with high throughput sequencing is rapidly expanding our knowledge of plant-associated virus diversity. Applying such approaches, we have identified five novel circular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA viruses from Poaceae and Apiaceae plant from South Africa and New Zealand. These viruses have a simple genomic organization, including two open reading frames that likely encode a Rep and a capsid protein (CP), a conserved nonanucleotide motif on the apex of a putative stem loop structure, and conserved rolling-circle replication and helicase motifs within their likely Rep: all suggesting that they replicate through rolling-circle replication. The Reps and the CPs putatively encoded by these five novel viruses share low to moderate degrees of similarity (22.1 - 44.6%) with other CRESS DNA viruses.

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