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Structural study of the membrane protein MscL using cell-free expression and solid-state NMR

Archive ouverte | Abdine, Alaa | CCSD

International audience

Cell-free Membrane Protein Expression for Solid-State NMR

Archive ouverte | Abdine, Alaa | CCSD

International audience. Although cell-free expression is a relative newcomer to the biochemical toolbox, it has already been reviewed extensively, even in the more specialized cases such as membrane protein expressi...

New labeling strategies for a structural study of the mechanosensitive channel of large conductance using cell-free synthesis. Développement et applications de protocoles de synthèse in vitro du canal mécanosensible bactérien à large conductance, pour son étude structurale par résonance magnétique nucléaire en phase solide

Archive ouverte | Abdine, Alaa | CCSD

Membrane proteins account for almost 30% of the proteome and play essential roles in many cellular mechanisms. The mechanosensitive channel of large conductance MscL is an intrinsic membrane protein of the inner membrane of Escher...

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