Staphylococcus aureus Small Colony Variants (SCVs): News From a Chronic Prosthetic Joint Infection

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Loss, Guilherme | Martins Simões, Patrícia | Valour, Florent | Farrel Cortês, Marina | Gonzaga, Luiz | Bergot, Marine | Trouillet-Assant, Sophie | Josse, Jérôme | Diot, Alan | Ricci, Emiliano | Vasconcelos, Ana Tereza | Laurent, Frédéric

Edité par CCSD ; Frontiers -

International audience. Small colony variants (SCV) of Staphylococcus aureus have been reported as implicated in chronic infections. Here, we investigated the genomic and transcriptomic changes involved in the evolution from a wild-type to a SCV from in a patient with prosthetic joint infection relapse. The SCV presented a stable phenotype with no classical auxotrophy and the emergence of rifampicin resistance. Whole Genome Sequencing (WGS) analysis showed only the loss of a 42.5 kb phage and 3 deletions, among which one targeting the rpoB gene, known to be the target of rifampicin and to be associated to SCV formation in the context of a constitutively active stringent response. Transcriptomic analysis highlighted a specific signature in the SCV strain including a complex, multi-level strategy of survival and adaptation to chronicity within the host including a protection from the inflammatory response, an evasion of the immune response, a constitutively activated stringent response and a scavenging of iron sources.

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