Maturation of pre‐40S particles in yeast and humans

Archive ouverte

Cerezo, Emilie | Plisson‐chastang, Célia | Henras, Anthony, K | Lebaron, Simon | Gleizes, Pierre‐emmanuel | O'Donohue, Marie-Françoise | Romeo, Yves | Henry, Yves

Edité par CCSD ; Wiley -

International audience. The synthesis of ribosomal subunits in eukaryotes requires the interplay of numerous maturation and assembly factors (AFs) that intervene in the insertion of ribo-somal proteins within pre-ribosomal particles, the ribosomal subunit precursors, aswell as in pre-ribosomal RNA (rRNA) processing and folding. Here, we review theintricate nuclear and cytoplasmic maturation steps of pre-40S particles, the precur-sors to the small ribosomal subunits, in both yeast and human cells, with particularemphasis on the timing and mechanisms of AF association with and dissociationfrom pre-40S particles and the roles of these AFs in the maturation process. Wehighlight the particularly complex pre-rRNA processing pathway in human cells,compared to yeast, to generate the mature 18S rRNA. We discuss the informationgained from the recently published cryo-electron microscopy atomic models ofyeast and human pre-40S particles, as well as the checkpoint/quality control sys-tems that seem to operate to probe functional sites within yeast cytoplasmic pre-40S particles.

• Description
• Sujet/Public
• Outil
• Outil d'anticipation
• Mémoire et thèse
• Gestion