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An embryonic CaVβ1 isoform promotes muscle mass maintenance via GDF5 signaling in adult mouse

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Traoré, Massiré | Gentil, Christel | Benedetto, Chiara | Hogrel, Jean-Yves | de La Grange, Pierre | Cadot, Bruno | Benkhelifa-Ziyyat, Sofia | Julien, Laura | Lemaitre, Mégane | Ferry, Arnaud | Pietri-Rouxel, France | Falcone, Sestina

Edité par CCSD ; American Association for the Advancement of Science (AAAS) -

International audience. Deciphering the mechanisms that govern skeletal muscle plasticity is essential to understand its pathophysiological processes, including age-related sarcopenia. The voltage-gated calcium channel CaV1.1 has a central role in excitation-contraction coupling (ECC), raising the possibility that it may also initiate the adaptive response to changes during muscle activity. Here, we revealed the existence of a gene transcription switch of the CaV1.1  subunit (CaV1) that is dependent on the innervation state of the muscle in mice. In a mouse model of sciatic denervation, we showed increased expression of an embryonic isoform of the subunit that we called CaV1E. CaV1E boosts downstream growth differentiation factor 5 (GDF5) signaling to counteract muscle loss after denervation in mice. We further reported that aged mouse muscle expressed lower quantity of CaV1E compared with young muscle, displaying an altered GDF5-dependent response to denervation. Conversely, CaV1E overexpression improved mass wasting in aging muscle in mice by increasing GDF5 expression. We also identified the human CaV1E analogous and show a correlation between CaV1E expression in human muscles and age-related muscle mass decline. These results suggest that strategies targeting CaV1E or GDF5 might be effective in reducing muscle mass loss in aging.

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