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In vitro biocompatibility of a dentine substitute cement on human MG63 osteoblasts cells: Biodentine™ versus MTA ®
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The authors also wish to express their appreciation to Beatrice Burdin, PhD, at the Microstructures Technology Center of University Claude Bernard Lyon1 for assistance with the SEM study. The AFM study was supported by the Characterization of Interactions Platform of the Nanobio Program, Grenoble University. We gratefully acknowledge the assistance on the English checking from Dr Huw Jones BSc PhD MRSC, Senior Lecturer in Chemistry for Environmental Science and Public Health, Middlesex University (UK).. International audience. AimTo compare the in vitro biocompatibility of Biodentine and White ProRoot((R)) mineral trioxide aggregate (MTA((R))) with MG63 osteoblast-like cells and to characterize the cement surface. MethodologyA direct contact model for MG63 osteoblast-like cells with cements was used for 1, 3 and 5days. Four end-points were investigated: (i) cement surface characterization by atomic force microscopy (AFM), (ii) cell viability by MTT assay, (iii) protein amount quantification by Bradford assay and (iv) cell morphology by SEM. Statistical analyses were performed by analysis of variance (anova) with a repetition test method. ResultsThe roughness of the cements was comparable as revealed by AFM analysis. The MTT test for Biodentine was similar to that of MTA((R)). Biodentine and MTA((R)) induced a similar but slight decrease in metabolic activity. The amount of total protein was significantly enhanced at day three (P<0.05) but slightly decreased at day five for both tested samples. Biodentine was tolerated as well as MTA((R)) in all cytotoxicity assays. SEM observations showed improvement of cell attachment and proliferation on both material surfaces following the three incubation periods. ConclusionThe biocompatibility of Biodentine to bone cells was comparable to MTA((R)).
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