ARTICLE RST1 and RIPR connect the cytosolic RNA exosome to the Ski complex in Arabidopsis

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Lange, Heike | Ndecky, Simon, y A | Gomez-Diaz, Carlos | Pflieger, David | Butel, Nicolas | Zumsteg, Julie | Kuhn, Lauriane | Piermaria, Christina | Chicher, Johana | Christie, Michael | Karaaslan, Ezgi, S | Lang, Patricia, L M | Weigel, Detlef | Vaucheret, Hervé | Hammann, Philippe | Gagliardi, Dominique

Edité par CCSD ; Nature Publishing Group -

International audience. The RNA exosome is a key 3'−5' exoribonuclease with an evolutionarily conserved structure and function. Its cytosolic functions require the co-factors SKI7 and the Ski complex. Here we demonstrate by co-purification experiments that the ARM-repeat protein RESURRECTION1 (RST1) and RST1 INTERACTING PROTEIN (RIPR) connect the cytosolic Arabidopsis RNA exosome to the Ski complex. rst1 and ripr mutants accumulate RNA quality control siRNAs (rqc-siRNAs) produced by the post-transcriptional gene silencing (PTGS) machinery when mRNA degradation is compromised. The small RNA populations observed in rst1 and ripr mutants are also detected in mutants lacking the RRP45B/CER7 core exosome subunit. Thus, molecular and genetic evidence supports a physical and functional link between RST1, RIPR and the RNA exosome. Our data reveal the existence of additional cytosolic exosome co-factors besides the known Ski subunits. RST1 is not restricted to plants, as homologues with a similar domain architecture but unknown function exist in animals, including humans.

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